Evaluation of Assay Methods for Hepatitis-Associated Antigen-Reference-Cited by-同舟云学术

Evaluation of Assay Methods for Hepatitis-Associated Antigen

Published:1972-06 Issue:6 Volume:23 Page:1037-1046
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Kissling Robert E.¹²,Barker Lewellys F.¹²

Affiliation:

1. Center for Disease Control, Atlanta, Georgia 30333

2. Division of Biologics Standards, National Institutes of Health, Bethesda, Maryland 20014

Abstract

Assay methods for hepatitis-associated antigen (HAA) were evaluated for sensitivity, or reproducibility, or both in a series of three trials in which both research and service-oriented laboratories participated. Agar-gel diffusion (AGD) methods were found to be the least sensitive and reproducible of the commonly employed assay methods. Complement fixation (CF) tests were consistently more sensitive than either AGD or counterelectrophoresis (CEP) methods for detection of HAA. With judicious choice of the antibody reagent, sensitivity of CEP techniques was equivalent to CF methods of HAA detection. None of the three major assay methods (AGD, CEP, or CF) compared in this study were capable of consistently detecting HAA when it was present in relatively low concentrations in human serum.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.23.6.1037-1046.1972

Reference15 articles.

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1. Platelet aggregation by hepatitis B surface antigen-antibody complexes;Infection and Immunity;1976-09

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