Targeted Transduction via CD4 by a Lentiviral Vector Uses a Clathrin-Mediated Entry Pathway-Reference-Cited by-同舟云学术

Targeted Transduction via CD4 by a Lentiviral Vector Uses a Clathrin-Mediated Entry Pathway

Published:2009-12-15 Issue:24 Volume:83 Page:13026-13031
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Liang Min¹²,Morizono Kouki¹²,Pariente Nonia¹²,Kamata Masakazu¹²,Lee Benhur¹²,Chen Irvin S. Y.¹²³

Affiliation:

1. Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, California

2. University of California—Los Angeles AIDS Institute, David Geffen School of Medicine, University of California, Los Angeles, California

3. Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, California

Abstract

ABSTRACT We recently developed a novel targeting Sindbis virus envelope pseudotyped lentiviral vector, 2.2ZZ, which acquires specific transduction capacity by antibody conjugation and binding with specific antigens on the surface of targeted cells. Here we characterize the virological properties of this vector by examining its targeting to CD4 antigen. Our results show that entry is dependent on CD4 cell surface density and occurs via the clathrin-mediated endocytic pathway. These findings provide insight into the mechanism of infection by a new viral vector with combined properties of Sindbis virus and lentiviruses and infectivity conferred by monoclonal antibody-ligand interactions.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.01530-09

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