Mutator Phenotype Resulting from DNA Polymerase IV Overproduction in Escherichia coli : Preferential Mutagenesis on the Lagging Strand-Reference-Cited by-同舟云学术

Mutator Phenotype Resulting from DNA Polymerase IV Overproduction in Escherichia coli : Preferential Mutagenesis on the Lagging Strand

Published:2005-10 Issue:19 Volume:187 Page:6862-6866
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Kuban Wojciech¹,Banach-Orlowska Magdalena¹,Bialoskorska Malgorzata¹,Lipowska Aleksandra¹,Schaaper Roel M.²,Jonczyk Piotr¹,Fijalkowska Iwona J.¹

Affiliation:

1. Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02 106 Warsaw, Poland

2. Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709

Abstract

ABSTRACT We investigated the mutator effect resulting from overproduction of Escherichia coli DNA polymerase IV. Using lac mutational targets in the two possible orientations on the chromosome, we observed preferential mutagenesis during lagging strand synthesis. The mutator activity likely results from extension of mismatches produced by polymerase III holoenzyme.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.187.19.6862-6866.2005

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