Molecular cloning of genes from Ruminococcus flavefaciens encoding xylanase and beta(1-3,1-4)glucanase activities

Author:

Flint H J1,McPherson C A1,Bisset J1

Affiliation:

1. Nutrition Division, Rowett Research Institute, Bucksburn, Aberdeen, United Kingdom.

Abstract

Clones expressing activity against xylan or beta(1-3,1-4)glucan (lichenan) were isolated from a library of Ruminococcus flavefaciens 17 DNA made in bacteriophage lambda EMBL3. Hybridization analyses indicated the recovery of four separate genes encoding xylanases that showed no detectable associated carboxylmethylcellulase activity. One of these genes was associated with clones that also expressed beta(1-3,1-4)glucanase and beta-xylosidase activities.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference22 articles.

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3. Bryant M. P. 1986. Genus Ruminococcus Sijpesteijn 1948 p. 1093-1097. In P. H. A. Sneath M. S. Moir M. E. Sharpe and J. G. Holt (ed.) Bergey's manual of systematic bacteriology vol. 2. The Williams & Wilkins Co. Baltimore.

4. Plasmidassociated transfer of tetracycline resistance in Bacteroides ruminicola;Flint H. J.;Appl. Environ. Microbiol.,1988

5. Lambda replacement vectors carrying polylinker sequences;Frischauf A.;J. Mol. Biol.,1983

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