Affiliation:
1. Laboratoire de Pathologie Végétale, Institut National de la Recherche Agronomique, Paris, France.
Abstract
The role of endoglucanases (endoglucanases Z and Y) in Erwinia chrysanthemi pathogenicity on Saintpaulia ionantha was assessed by mutagenizing cloned cel genes (celZ and celY) and recombining them with the chromosomal alleles. Strains with an omega interposon in celZ, a deletion in celY, or a double cel mutant were as virulent as the wild-type strain. However, in the strain with a deletion in celY, a delay in the appearance of symptoms was observed, and then maceration progressed as in plants infected with the wild-type strain, suggesting that E. chrysanthemi endoglucanases play a minor role in soft rot disease development.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference20 articles.
1. Aymeric J.-L. C. Enard F. Renou C. Neema M. Boccara and D. Expert. 1990. The requirement of depolymerizing enzymes and a high affinity iron transport system for pathogenicity of Erwinia chrysanthemi p. 679-685. Proceeding of the 7th International Conference on Plant Pathogenic Bacteria.
2. Mapping and regulation of the cel genes in Erwinia chrysanthemi;Aymeric J.;Mol. Gen. Genet.,1988
3. .Barras F. Personal communication.
4. Pathogenic behaviour of pectinase-defective Erwinia chrysanthemi mutants on different plants: possible involvement of pectic enzymes in host specificity;Beaulieu C.;Mol. Plant-Microbe Interact.,1993
5. Detection of depolymerase enzymes after electrophoresis or electrofocusing, or in titration curves;Bertheau Y.;Anal. Biochem.,1984
Cited by
19 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献