Nucleotide metabolism in Lactococcus lactis: salvage pathways of exogenous pyrimidines

Author:

Martinussen J1,Andersen P S1,Hammer K1

Affiliation:

1. Center for Lactic Acid Bacteria, Technical University of Denmark, Lyngby.

Abstract

By measuring enzyme activities in crude extracts and studying the effect of toxic analogs (5-fluoropyrimidines) on cell growth, the metabolism of pyrimidines in Lactococcus lactis was analyzed. Pathways by which uracil, uridine, deoxyuridine, cytidine, and deoxycytidine are metabolized in L. lactis were established. They are similar to those found in Escherichia coli except that lactococci are unable to utilize cytosine.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference16 articles.

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2. Taxonomic differentiation of bacteriophages of Lactococcus lactis by electron microscopy, DNA-DNA hybridization and protein profiles;Braun V.;J. Gen. Microbiol.,1989

3. Two plasmid-determined restriction and modification systems in Streptococcus lactis;Chopin A. M.;Plasmid,1984

4. Cytidine deaminase from Escherichia coli. Purification, properties, and inhibition by the potential transition state analog 3,4,5,6-tetrahydrouridine;Cohen R. M.;J. Biol. Chem.,1971

5. The mode of action of 5-fluorouracil and its derivatives;Cohen S. S.;Proc. Natl. Acad. Sci. USA,1958

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