Affiliation:
1. Department of Epidemiology, School of Public Health, University of North Carolina, Chapel Hill 27599-7400.
Abstract
We examined the heat shock response of the pathogenic spirochetes Treponema pallidum, Borrelia burgdorferi, and Leptospira interrogans and certain saprophytic spirochetes. Cellular proteins synthesized after shifts to higher temperatures were [35S]methionine labeled and analyzed by gel electrophoresis and fluorography. Only T. pallidum failed to exhibit an obvious heat shock response. GroEL and DnaK homologs were identified in the various species, although these proteins were not thermoinducible in T. pallidum or Treponema denticola. DNA hybridization studies indicate that spirochetal groEL and dnaK genes are highly conserved.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference31 articles.
1. Isolation and cultivation of Lyme disease spirochetes;Barbour A. G.;Yale J. Biol. Med.,1984
2. Antigenic variation of a relapsing fever Borrelia species. Annu;Barbour A. G.;Rev. Micrbiol.,1990
3. Protein synthesis by Treponemna pallidium extracted from infected rabbit tissue;Baseman J. B.;Infect. Immun.,1974
4. Interaction of Hsp 7O with newly synthesized proteins: implications for protein folding and assembly;Beckmann R. P.;Science,1990
5. The inadequacy of a single prolonged fever for the treatment of early acute syphilis;Boak R. A.;Am. J. Syph. Gonor. Vener. Dis.,1942
Cited by
69 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献