Quantitation of intracellular zidovudine phosphates by use of combined cartridge-radioimmunoassay methodology-Reference-Cited by-同舟云学术

Quantitation of intracellular zidovudine phosphates by use of combined cartridge-radioimmunoassay methodology

Published:1996-11 Issue:11 Volume:40 Page:2651-2654
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Robbins B L¹,Waibel B H¹,Fridland A¹

Affiliation:

1. Department of Infectious Disease, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.

Abstract

This report describes the development of a potentially clinical method to measure the cellular metabolites of zidovudine (ZDV) in patients receiving the drug. This new method combines the use of Sep-Pak cartridges to separate ZDV phosphates with radioimmunoassaying to quantitate ZDV. The detection limit is 0.02 pmol/10(6) cells, and this assay can measure a wide range of intracellular drug concentrations. The use of the cartridge-radioimmunoassay methodology should prove very useful for in vivo cellular pharmacokinetic studies of ZDV.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.40.11.2651

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