In Vivo Transduction with Shiga Toxin 1-Encoding Phage-Reference-Cited by-同舟云学术

In Vivo Transduction with Shiga Toxin 1-Encoding Phage

Published:1998-09 Issue:9 Volume:66 Page:4496-4498
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Acheson David W. K.¹,Reidl Joachim²,Zhang Xiaoping¹,Keusch Gerald T.¹,Mekalanos John J.³,Waldor Matthew K.¹

Affiliation:

1. Division of Geographic Medicine and Infectious Diseases and Tupper Research Institute, Tufts University School of Medicine,1 and

2. Research Center for Infectious Disease, University of Wuerzburg, Wuerzburg, Germany2

3. Department of Microbiology and Molecular Genetics and the Shipley Institute of Medicine, Harvard Medical School,3 Boston, Massachusetts, and

Abstract

ABSTRACT To facilitate the study of intestinal transmission of the Shiga toxin 1 (Stx1)-converting phage H-19B, Tn 10d-bla mutagenesis of an Escherichia coli H-19B lysogen was undertaken. Two mutants containing insertions in the gene encoding the A subunit of Stx1 were isolated. The resultant ampicillin-resistant E. coli strains lysogenic for these phages produced infectious H-19B particles but not active toxin. These lysogens were capable of transducing an E. coli recipient strain in the murine gastrointestinal tract, thereby demonstrating that lysogens of Shiga toxin-converting phages give rise to infectious virions within the host gastrointestinal tract.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.66.9.4496-4498.1998

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