Author:
Wills J W,Lasker B A,Sirotkin K,Riggsby W S
Abstract
We report the isolation and analysis of the rapidly reassociating DNA of the pathogenic, dimorphic fungus Candida albicans. Minicot analysis of whole-cell repetitive DNA suggested that a significant portion of this component was mitochondrial DNA. Genomic blot hybridizations in which radioactive whole-cell repetitive DNA was used as a probe revealed eight major EcoRI bands in the molecular weight range resolved by the gel system used. Isolation and analysis of high-purity mitochondrial DNA have shown that five of these bands are of mitochondrial origin. The remaining three bands are of nuclear origin and represent repetitive sequences that are found in the nuclear genome. Attempts to isolate nuclear DNA that was completely free of mitochondrial DNA contamination were unsuccessful.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
44 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献