Affiliation:
1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
2. OIE Reference Laboratory for Swine Streptococcosis, Nanjing, China
3. Key Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing, China
Abstract
ABSTRACT
Streptococcus suis
has received increasing attention for its involvement in severe human infections worldwide as well as in multidrug resistance. Two-component signaling systems (TCSSs) play important roles in bacterial adaptation to various environmental stimuli. In this study, we identified a novel TCSS located in
S. suis
serotype 2 (SS2), designated VraSR
SS
, which is involved in bacterial pathogenicity and susceptibility to antimicrobials. Our data demonstrated that the
yvqF
SS
gene, located upstream of
vraSR
SS
, shared the same promoter with the TCSS genes, which was directly regulated by VraSR
SS
, as shown in electrophoretic mobility shift assays. Notably, YvqF
SS
and VraSR
SS
constitute a novel multidrug resistance module of SS2 that participates in resistance to certain groups of antimicrobials. Further analyses showed that VraSR
SS
inactivation significantly attenuated bacterial virulence in animal models, which, coupled with the significant activation of VraSR
SS
expression observed in host blood, strongly suggested that VraSR
SS
is an important regulator of SS2 pathogenicity. Indeed, RNA-sequencing analyses identified 106 genes that were differentially expressed between the wild-type and Δ
vraSR
SS
strains, including genes involved in capsular polysaccharide (CPS) biosynthesis. Subsequent studies confirmed that VraSR
SS
indirectly regulated the transcription of CPS gene clusters and, thus, controlled the CPS thickness shown by transmission electron microscopy. Decreased CPS biosynthesis caused by
vraSR
SS
deletion subsequently increased bacterial adhesion to epithelial cells and attenuated antiphagocytosis against macrophages, which partially clarified the pathogenic mechanism mediated by VraSR
SS
. Taken together, our data suggest that the novel TCSS, VraSR
SS
, plays critical roles for multidrug resistance and full virulence in SS2.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
17 articles.
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