Affiliation:
1. Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
Abstract
ABSTRACT
Salmonella enterica
subsp.
enterica
serovar Enteritidis is a leading causative agent of gastroenteritis in humans. This pathogen also colonizes the intestinal tracts of poultry and can spread systemically in chickens. Transfer to humans usually occurs through undercooked or improperly handled poultry meat or eggs. The bacterial twin-arginine transport (Tat) pathway is responsible for the translocation of folded proteins across the cytoplasmic membrane. In order to study the role of the Tat system in the infection and colonization of chickens by
Salmonella
Enteritidis, we constructed chromosomal deletion mutants of the
tatB
and
tatC
genes, which are essential components of the Tat translocon. We observed that the
tat
mutations affected bacterial cell morphology, motility, and sensitivity to albomycin, sodium dodecyl sulfate (SDS), and EDTA. In addition, the mutant strains showed reduced invasion of polarized Caco-2 cells. The wild-type phenotype was restored in all our
Salmonella
Enteritidis
tat
mutants by introducing episomal copies of the
tatABC
genes. When tested in chickens by use of a
Salmonella
Enteritidis Δ
tatB
strain, the Tat system inactivation did not substantially affect cecal colonization, but it delayed systemic infection. Taken together, our data demonstrated that the Tat system plays a role in
Salmonella
Enteritidis pathogenesis.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
32 articles.
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