Development and Characterization of a Xylose-Inducible Gene Expression System for Clostridium perfringens-Reference-Cited by-同舟云学术

Development and Characterization of a Xylose-Inducible Gene Expression System for Clostridium perfringens

Published:2011-12 Issue:23 Volume:77 Page:8439-8441
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Nariya Hirofumi¹,Miyata Shigeru²,Kuwahara Tomomi¹,Okabe Akinobu³

Affiliation:

1. Department of Microbiology, Faculty of Medicine, Kagawa University, 1750-1 Miki-cho, Kita-gun, Kagawa 761-0793, Japan

2. Department of Food and Nutritional Sciences, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, Japan

3. Department of Human Nutrition, Faculty of Contemporary Life Science, Chugokugakuen University, 83 Niwase, Kita-ku, Okayama 701-0197, Japan

Abstract

ABSTRACT A xylose-inducible gene expression vector for Clostridium perfringens was developed. Plasmid pXCH contains a chromosomal region from Clostridium difficile ( xylR -P xy lB ): xylR , encoding the xylose repressor, xylO , the xyl operator sequence, and P xylB , the divergent promoter upstream of xylBA encoding xylulo kinase and xylose isomerase. pXCH allows tightly regulated expression of the chloramphenicol acetyltransferase reporter and the α-toxin genes in response to the inducer concentration. Thus, pXCH could constitute a new valuable genetic tool for study of C. perfringens .

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.05668-11

Reference16 articles.

1. Development and Characterization of a Xylose-Dependent System for Expression of Cloned Genes in Bacillus subtilis : Conditional Complementation of a Teichoic Acid Mutant

2. Transcription of the xyl Operon is Controlled in Bacillus subtilis by Tandem Overlapping Operators Spaced by four Base-pairs

3. Construction and Characterization of a Lactose-Inducible Promoter System for Controlled Gene Expression in Clostridium perfringens

4. A novel type of DNA curvature present in a Clostridium perfringens ferredoxin gene: characterization and role in gene expression

5. Absolute and relative QPCR quantification of plasmid copy number in Escherichia coli

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