Transcriptional analysis of the Streptomyces glaucescens tetracenomycin C biosynthesis gene cluster-Reference-Cited by-同舟云学术

Transcriptional analysis of the Streptomyces glaucescens tetracenomycin C biosynthesis gene cluster

Published:1993-06 Issue:12 Volume:175 Page:3887-3892
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Decker H¹,Hutchinson C R¹

Affiliation:

1. School of Pharmacy, University of Wisconsin, Madison 53706.

Abstract

A 12.6-kb DNA fragment from Streptomyces glaucescens GLA.0 containing the 12 genes for tetracenomycin (TCM) C biosynthesis and resistance enabled Streptomyces lividans to produce TCM C. Transcriptional analysis of the tcmPG intergenic region in this cluster established the presence of two divergent promoters. The tcmIc mutation, a T-to-G transversion in the -10 region of the tcmG promoter, decreased promoter activity drastically at the stationary growth stage and time of maximum TCM C accumulation. This promoter may direct the transcription of a tcmGHIJKLMNO operon, while the other promoter is for tcmP.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.175.12.3887-3892.1993

Reference44 articles.

1. Transcript mapping using [35S]-DNA probes, trichloracetate solvent and dideoxy sequencing ladders: a rapid method for identification of transcriptional start points;Aldea M.;Gene,1988

2. Analysis of the nucleotide sequence of the Streptomyces glaucescens tcmI genes provides key information about the enzymology of polyketide antibiotic biosynthesis;Bibb M. J.;EMBO J.,1989

3. Bibb M. J. and G. R. Janssen. 1987. Unusual features of transcription and translation of antibiotic resistance genes in antibiotic-producing Streptomyces p. 309-318. In M. Alacevic D. H4ranueli and Z. Toman (ed.) Genetics of industrial microorganisms: proceedings of the Fifth International Symposium. Pliva Zagreb Yugoslavia.

4. Cloning and analysis of the promoter region of the erythromycin-resistance gene (ermE) of Streptomyces erythraeus;Bibb M. J.;Gene,1985

5. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding;Bradford M. M.;Anal. Biochem.,1976

Cited by 33 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Advancement of Biotechnology by Genetic Modifications;Methods in Molecular Biology;2018

2. The complete genome sequence of the actinobacterium Streptomyces glaucescens GLA.O (DSM 40922) carrying gene clusters for the biosynthesis of tetracenomycin C, 5`-hydroxy streptomycin, and acarbose;Journal of Biotechnology;2017-11

3. Essential Role of Genetics in the Advancement of Biotechnology;Microbial Carotenoids From Fungi;2012

4. Type II PKS;Comprehensive Natural Products II;2010

5. High titer production of tetracenomycins by heterologous expression of the pathway in a Streptomyces cinnamonensis industrial monensin producer strain;Metabolic Engineering;2009-11