Bovine pulmonary endothelial cell damage mediated by Pasteurella haemolytica pathogenic factors

Author:

Breider M A1,Kumar S1,Corstvet R E1

Affiliation:

1. Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, Knoxville 37901.

Abstract

The in vitro effects of Pasteurella haemolytica components on bovine pulmonary endothelial monolayers were investigated to determine the relative role of individual bacterial factors in the pathogenesis of bovine pulmonary pasteurellosis. Bovine pulmonary endothelial monolayers were treated with P. haemolytica bacterial culture supernatant (CS) and P. haemolytica lipopolysaccharide. At 22 h postinoculation, the CS produced severe damage to the endothelial cells, indicated by high 51Cr release, extensive cellular detachment, and morphologic changes characterized by cell contraction, cytoplasmic blebbing, and loss of monolayer confluency. The neutralization of leukotoxin activity of the CS by heat inactivation was ineffective in decreasing the damage to endothelial cells; however, leukotoxin-neutralizing monoclonal antibody slightly diminished the toxic effect. P. haemolytica lipopolysaccharide by itself or as a supplement to CS produced endothelial cell damage similar to that of CS. The preincubation of CS dilutions (10(-1) and 10(-2)) or P. haemolytica lipopolysaccharide with polymyxin B almost completely eliminated cell toxicity. These studies show that P. haemolytica produces a soluble factor that is consistent with bacterial lipopolysaccharide and that is directly toxic to bovine pulmonary endothelial cells in vitro.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference19 articles.

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2. Pulmonary lesions induced by Pasteurella haemolytica in neutrophil sufficient and neutrophil deficient calves;Breider M. A.;Can. J. Vet. Res.,1988

3. Inhibition of endotoxin induced cytotoxicity and prostonoid production in cultured bovine pulmonary endothelial cells by dimethyl sulfoxide and allopurinol;Bringham K. L.;J. Appl. Physiol.,1987

4. Effects of Pasteurella haemolytica lipopolysaccharide in selected functions of bovine leukocytes;Confer A. W.;Am. J. Vet. Res.,1986

5. Acute inflammation and microthrombosis induced by endotoxin, interleukin-1, and tumor necrosis factor and their implication in gram-negative infection;Cybulsky M. I.;Lab. Invest.,1988

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