Evaluation of the API 20E system for identification of nonfermentative Gram-negative bacteria

Author:

Shayegani M,Maupin P S,McGlynn D M

Abstract

The API 20E system for Enterobacteriaceae, recently broadened to include identification of nonfermentative gram-negative bacteria, was evaluated and compared with the conventional method for complete identification of 221 nonfermenters, which were well distributed into 48 species or biotypes and included organisms not listed in the API 20E data base. The results of 16 tests common to both systems were in close agreement. The API 20E system correctly identified 71 (43%) of the 165 organisms included in the API 20E data base. However, almost 90% of Acinetobacter calcoaceticus, three species of Pseudomonas, and Bordetella bronchiseptica were correctly identified to species.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference13 articles.

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2. Buchanan R. E. and N. E. Gibbons (ed.). 1974. Bergey's manual of determinative bacteriology 8th ed. The Williams and Wilkins Co. Baltimore Md.

3. Evaluation of two rapid methods for identification of commonly encountered nonfermenting or oxidase-positive gram-negative rods;Dowda H.;J. Clin. Microbiol.,1977

4. Hugh R. and G. L Gilardi. 1974. Pseudomonas p. 250-269. In E. H. Lennette E. H. Spaulding and J. P. Truant (ed.) Manual of clinical microbiology 2nd ed. American Society for Microbiology Washington D.C.

5. Evaluation of five test kits API, Auxo Tab, Enterotube, Patho Tech and R/B for identification of Enterobacteriaceae;Nord C. E.;Med. Microbiol. Immunol.,1974

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