Endocarditis Caused by Culture-Negative Organisms Visible by Brown and Brenn Staining: Utility of PCR and DNA Sequencing for Diagnosis-Reference-Cited by-同舟云学术

Endocarditis Caused by Culture-Negative Organisms Visible by Brown and Brenn Staining: Utility of PCR and DNA Sequencing for Diagnosis

Published:2001-05 Issue:5 Volume:39 Page:2025-2027
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Wilck Marissa B.¹,Wu Yanyun¹,Howe John G.¹,Crouch Jill Y.¹,Edberg Stephen C.¹²

Affiliation:

1. Departments of Laboratory Medicine1and

2. Internal Medicine,2 Yale University School of Medicine, New Haven, Connecticut

Abstract

ABSTRACT Two cases of culture-negative endocarditis with cocci seen in valve vegetations are presented. The organisms were identified by molecular analysis using broad-range PCR primers complementary to the 16S rRNA gene, sequencing, and database search using BLAST software. The results and utility of this method are discussed.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.5.2025-2027.2001

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