Real-Time PCR Assay Targeting IS 481 of Bordetella pertussis and Molecular Basis for Detecting Bordetella holmesii-Reference-Cited by-同舟云学术

Real-Time PCR Assay Targeting IS 481 of Bordetella pertussis and Molecular Basis for Detecting Bordetella holmesii

Published:2001-05 Issue:5 Volume:39 Page:1963-1966
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Reischl Udo¹,Lehn Norbert¹,Sanden Gary N.²,Loeffelholz Mike J.³

Affiliation:

1. Institute of Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg, Germany1;

2. Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303332; and

3. State Hygienic Laboratory, University of Iowa, Iowa City, Iowa 522423

Abstract

ABSTRACT Detection of Bordetella holmesii by a real-time PCR assay targeting IS 481 of Bordetella pertussis is reported. Sequencing of IS 481 -specific PCR products from B. pertussis and B. holmesii isolates revealed sequence homology. Restriction fragment length polymorphism demonstrated a low copy number of IS 481 -like sequences in B. holmesii . These results, and culture of B. holmesii from patients with cough, suggest that the specificity and predictive value of IS 481 -based PCR assays for pertussis may be compromised.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.5.1963-1966.2001

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