Affiliation:
1. Unité des Rickettsies, CNRS UPRESA 6020, Faculté de Médecine, 13385 Marseille Cedex 05, France
Abstract
ABSTRACT
As part of a survey for trench fever among homeless people in Marseilles, France, we attempted isolation of
Bartonella quintana
from body lice. A decontamination protocol of immersion in 70% ethanol with 0.2% iodine was devised and was tested with a laboratory colony of body lice. Lice which had been experimentally contaminated with either
Escherichia coli, Staphylococcus epidermidis
, or
Acinetobacter
spp. were successfully decontaminated, and this process did not prevent the culture of
B. quintana
from these lice. One hundred sixty-one lice obtained from homeless patients were studied by the protocol.
B. quintana
was isolated on axenic medium from 15 of 161 body lice and was detected in 41 of 161 lice by PCR.
Acinetobacter
spp. and
Serratia marcescens
were also isolated from body lice. The sensitivities of PCR and culture of
B. quintana
were 98 and 36%, respectively. These procedures may be useful for epidemiologic studies of trench fever and for the recovery of strains for characterization and comparison.
Publisher
American Society for Microbiology
Cited by
59 articles.
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