Evaluation of PCR Using TRC 4 and IS 6110 Primers in Detection of Tuberculous Meningitis-Reference-Cited by-同舟云学术

Evaluation of PCR Using TRC 4 and IS 6110 Primers in Detection of Tuberculous Meningitis

Published:2001-05 Issue:5 Volume:39 Page:2006-2008
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Narayanan Sujatha¹,Parandaman Vijayalakshmi¹,Narayanan P. R.¹,Venkatesan P.¹,Girish C.²,Mahadevan S.²,Rajajee Sarala³

Affiliation:

1. Tuberculosis Research Centre, Chetput, Chennai 600 031,1

2. Department of Pediatrics, JIPMER, Pondicherry 605 006,2 and

3. Childs Trust Hospital, Chennai 600 034,3India

Abstract

ABSTRACT We have evaluated a new set of primers (TRC 4 ) in comparison with the IS 6110 primers commonly used in PCR to detect tuberculous meningitis among children. The levels of concordance between the results of IS 6110 PCR and TRC 4 PCR with cerebrospinal fluid specimens from patients with clinically confirmed tuberculous meningitis were 80 and 86%, respectively. Results with the two primer sets were concordant for 55 positive and 22 negative specimens ( n = 98). We conclude that the sensitivity of PCR can be increased by using both IS 6110 and TRC 4 primers.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.5.2006-2008.2001

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