Affiliation:
1. Department of Tumor Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
Abstract
The 5' splice site signal (5'ss) in Moloney murine sarcoma virus ts110 (MuSVts110) RNA was found to participate in the regulation of its splicing phenotype. This 5'ss (CAG/GUAGGA) departs from the mammalian consensus (CAG/GURAGU) at positions +4 and +6, both of which base pair with U1 and U6 small nuclear RNAs during splicing. A doubling in splicing efficiency and near elimination of the splicing thermosensitivity characteristic of MuSVts110 were observed in 5'ss mutants containing a U at position +6 (termed 5' A6U), even in those in which U1-5'ss complementarity had been reduced. At the permissive temperature (28 degrees C), the 5' A6U mutation increased the efficiency of the second splicing reaction, while at the nonpermissive temperature (39 degrees C), both splicing reactions were positively affected.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference39 articles.
1. Sequence requirements for splicing of higher eukaryotic nuclear pre-mRNA;Aebi M.;Cell,1986
2. Genetic selection for balanced retroviral RNA splicing: novel regulation involving the second step can be mediated by transitions in the polypyrimidine tract;Bouck J.;J. Virol.,1995
3. Exploitation of a thermosensitive splicing event to study pre-mRNA splicing in vivo;Cizdziel P. E.;Mol. Cell. Biol.,1988
4. The U1 small ribonucleoprotein/5~ splice site interaction affects U2AF65 binding to the 3~ splice site;Cote J.;J. Biol. Chem.,1995
5. Complementation by SR proteins of pre-mRNA splicing reactions depleted of U1 snRNP;Crispino J. D.;Science,1994
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献