Affiliation:
1. Education and Clinical Center, Veterans Affairs Medical Center, Gainesville, Florida 32608, USA. bbender@qm.server.ufl.edu
Abstract
Mice immunized with two intragastrically administered doses of a replication-deficient recombinant vaccinia virus containing the hemagglutinin and nucleoprotein genes from H1N1 influenza virus developed serum anti-H1 immunoglobulin G (IgG) antibody that completely protected the lungs from challenge with H1N1. Almost all of the mice given two intragastric doses also developed mucosal anti-H1 IgA antibody, and those with high anti-H1 IgA titers had completely protected noses. Intramuscular injection of the vaccine protected the lungs but not the noses from challenge. We also found that the vaccine enhanced recovery from infection caused by a shifted (H3N2) influenza virus, probably through the induction of nucleoprotein-specific cytotoxic T-lymphocyte activity. A replication-deficient, orally administered, enteric-coated, vaccinia virus-vectored vaccine might safely protect humans against influenza.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference73 articles.
1. Efficacy of influenza haemagglutinin and nucleoprotein as protective antigens against influenza virus infection in mice;Andrew M. E.;Scand. J. Immunol.,1988
2. .Bell W. P. A. Small Jr. and B. S. Bender. Unpublished data.
3. Class I major histocompatibility complex-restricted cytotoxic T-lymphocytes are not necessary for heterotypic immunity to influenza;Bender B. S.;J. Infect. Dis.,1994
4. Transgenic mice lacking class I major histocompatibility complex-restricted T-cells have delayed viral clearance and increased mortality after influenza virus challenge;Bender B. S.;J. Exp. Med.,1992
5. Bender B. S. M. P. Johnson C. Flexner B. Moss and P. A. Small Jr. 1990. Cytotoxic T-lymphocyte activity induced by influenza vaccination in young and aged mice p. 69-73. In F. Brown R. Chanock H. S. Ginsberg and R. A. Lerner (ed.) Vaccines 90. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Cited by
87 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献