Unique Properties of the Alpha-Helical DNA-Binding Protein KfrA Encoded by the IncU Incompatibility Group Plasmid RA3 and Its Host-Dependent Role in Plasmid Maintenance-Reference-Cited by-同舟云学术

Unique Properties of the Alpha-Helical DNA-Binding Protein KfrA Encoded by the IncU Incompatibility Group Plasmid RA3 and Its Host-Dependent Role in Plasmid Maintenance

Published:2021-01-04 Issue:2 Volume:87 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Lewicka Ewa¹,Mitura Monika¹,Steczkiewicz Kamil²,Kieracinska Justyna¹³,Skrzynska Kamila³,Adamczyk Malgorzata³,Jagura-Burdzy Grazyna¹^ORCID

Affiliation:

1. Institute of Biochemistry and Biophysics, Department of Microbial Biochemistry, PAS, Warsaw, Poland

2. Institute of Biochemistry and Biophysics, Department of Bioinformatics, PAS, Warsaw, Poland

3. Warsaw University of Technology, Faculty of Chemistry, Chair of Drug and Cosmetics Biotechnology, Warsaw, Poland

Abstract

Alpha-helical coiled-coil KfrA-type proteins are encoded by various broad-host-range low-copy-number conjugative plasmids. The DNA-binding protein KfrA encoded on the RA3 plasmid, a member of the IncU incompatibility group, oligomerizes, forms a complex with another plasmid-encoded, alpha-helical protein, KfrC, and interacts with the segrosome proteins IncC and KorB. The unique mode of KfrA dimer binding to the repetitive operator is required for a KfrA role in the stable maintenance of RA3 plasmid in distinct hosts.

Funder

National Science Centre (NCN) Poland

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01771-20

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