Affiliation:
1. Faculty of Veterinary Medicine, University of São Paulo, São Paulo, SP, Brazil
2. Faculty of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, MG, Brazil
3. Biological Institute, São Paulo, SP, Brazil
4. Department of Pathology, University of Texas Medical Branch, Galveston, Texas
Abstract
ABSTRACT
Free-living adult
Amblyomma incisum
ticks were collected in an Atlantic rainforest area at Intervales State Park, State of São Paulo, Brazil. From an
A. incisum
specimen, rickettsiae were successfully isolated in Vero cell culture by the shell vial technique. Rickettsial isolation was confirmed by optical microscopy, transmission electron microscopy, and PCRs targeting portions of the rickettsial genes
gltA
,
htrA
,
rrs
, and
sca1
on infected cells. Fragments of 1,089, 457, 1,362, and 443 nucleotides of the
gltA
,
htrA
,
rrs
, and
sca1
genes, respectively, were sequenced. By BLAST analysis, the partial sequence of
rrs
of the
A. incisum
rickettsial isolate was closest to the corresponding sequence of
Rickettsia bellii
(99.1% similarity). The
gltA
partial sequence was closest to the corresponding sequences of “
Candidatus
Rickettsia tarasevichiae” (96.1% similarity) and
Rickettsia canadensis
(95.8% similarity). The
htrA
partial sequence was closest to the corresponding sequence of
R. canadensis
(89.8% similarity). The
sca1
partial sequence was closest to the corresponding sequence of
R. canadensis
(95.2% similarity). Since our rickettsial isolate was genetically distinct from other
Rickettsia
species, we propose a new species designated
Rickettsia monteiroi
sp. nov. Phylogenetic analyses indicated that
R. monteiroi
belongs to the canadensis group within the genus
Rickettsia
, together with the species
R. canadensis
and “
Candidatus
R. tarasevichiae”. Little or no antibody cross-reaction was observed between sera of
R. monteiroi
-inoculated guinea pigs and
R. bellii
-,
Rickettsia rickettsii
-, or
R. canadensis
-inoculated guinea pigs.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
33 articles.
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