Immunoelectron-microscopic quantitation of differential levels of chlamydial proteins in a cell culture model of persistent Chlamydia trachomatis infection

Author:

Beatty W L1,Morrison R P1,Byrne G I1

Affiliation:

1. Department of Medical Microbiology and Immunology, University of Wisconsin, Madison 53706.

Abstract

Electron immunolabeling techniques were used for quantitative evaluation of alterations in the steady-state levels of chlamydial antigens in persistent Chlamydia trachomatis cultures. Gamma interferon-mediated persistent chlamydial development correlated with an increase in the levels of the chlamydial heat shock protein (hsp60) and with a significant reduction in the levels of the major outer membrane protein.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference11 articles.

1. Interferon--y in endocervical secretions of women infected with Chlamydia trachomatis;Arno J. N.;J. Infect. Dis.,1990

2. Mapping antigenic domains expressed by Chlamydia trachomatis major outer membrane protein genes;Baehr W.;Proc. Natl. Acad. Sci. USA,1988

3. Morphological and antigenic characterization of interferon--y mediated persistent Chlamydia trachomatis infection in vitro;Beatty W. L.;Proc. Natl. Acad. Sci. USA,1993

4. Beatty W. L. R P. Morrison and G. L Byrne. 1993. Characterization of long term chlamydial persistence and recovery of infectivity in cell culture D-151. Abstr. 93rd Gen. Meet. Am. Soc. Microbiol. 1993.

5. Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis;Caldwell H. D.;Infect. Immun.,1981

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