Protective effect of rSm28GST-specific T cells in schistosomiasis: role of gamma interferon

Author:

Pancre V1,Wolowczuk I1,Guerret S1,Copin M C1,Delanoye A1,Capron A1,Auriault C1

Affiliation:

1. Centre d'Immunologie et de Biologie Parasitaire, Institut National de la Santé et de la Recherche Médicale U 167, Institut Pasteur, Lille, France.

Abstract

Immunization with a single dose of 50 micrograms of recombinant Schistosoma mansoni 28-kDa glutathione-S-transferase (rSm28GST) was able to induce a reduction in the worm burden, the number of eggs, and the degree of hepatic fibrosis as quantified by the measurement of collagen content in the liver of S. mansoni-infected mice. No relationship was found between anti-Sm28GST immunoglobulin G and immunoglobulin A titers and the levels of protection obtained. Adoptive transfers of Sm28GST-specific total, CD4+, or CD8+ T cells reproduced the protective effect obtained with the recombinant molecule. Moreover, experiments studying in vivo T-cell depletion demonstrated that anti-CD4- or anti-CD8-treated mice showed a significant decrease in the protective effect conferred, suggesting a role of the two T-cell subpopulations in the expression of Sm28GST-mediated protection against hepatic damage. Sm28GST-specific cells produced little interleukin-4 and high levels of gamma interferon. Treatment of immunized mice with anti-gamma interferon antibody totally suppressed the Sm28GST-induced protective effect and led to the rapid death of infected animals, suggesting a role for this cytokine in the expression of the protective immunity obtained after immunization with rSm28GST.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference38 articles.

1. A purified 28,000 dalton protein from Schistosoma mansoni adult worms protects rats and mice against experimental schistosomiasis;Balloul J. M.;J. Immunol.,1987

2. Immunopathology of Schistosoma mansoni infection;Boros D. L.;Clin. Microbiol. Rev.,1989

3. Immunization of mice and baboons with the recombinant Sm 28GST affects both worm viability and fecundity after experimental infection with Schistosoma mansoni;Boulanger D.;Parasite Immunol.,1991

4. Modulation of granulomatous hypersensitivity. II. Participation of Ly 1+ and Ly 2+ T Iymphocytes in the suppression of granuloma formation and Iymphokine production in Schistosoma mansoni-infected mice;Chensue S. W.;J. Immunol.,1981

5. T Iymphocytes that contribute to the immunoregulation of granuloma formation in chronic murine schistosomiasis;Colley D. J.;J. Immunol.,1981

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