Shuttle mutagenesis of Legionella pneumophila: identification of a gene associated with host cell cytopathicity-Reference-Cited by-同舟云学术

Shuttle mutagenesis of Legionella pneumophila: identification of a gene associated with host cell cytopathicity

Published:1994-09 Issue:9 Volume:62 Page:4075-4080
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Arroyo J¹,Hurley M C¹,Wolf M¹,McClain M S¹,Eisenstein B I¹,Engleberg N C¹

Affiliation:

1. Department of Microbiology, University of Michigan Medical School, Ann Arbor 48109.

Abstract

We performed shuttle mutagenesis of Legionella pneumophila. Mutants were screened for reduced cellular infectivity. Approximately 10% of the mutants had decreased cytopathicity. The DNA sequence of one locus was determined; the inferred amino acid sequence revealed homology with transport proteins including Escherichia coli TolC, Bordetella pertussis CyaE, and Alcaligenes eutrophus CzcC and CnrC.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/iai.62.9.4075-4080.1994

Reference35 articles.

1. Albano M. A. Unpublished data.

2. PhoA gene fusions in Legionella pneumophila generated in vivo using a new transposon;Albano M. A.;MudphoA. Mol. Microbiol.,1992

3. Arber W. L. Enquist B. Hohn N. E. Murray and K. Murray. 1983. Experimental methods for use with lambda p. 433-451. In R. W. Hendrix J. W. Roberts F. W. Stahl and R. A. Weisberg (ed.) Lambda II. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.

4. Arroyo J. 1993. Ph.D. thesis. University of Michigan Ann Arbor.

5. Arroyo J. B. I. Eisenstein and N. C. Engleberg. 1993. Construction ofphoA gene fusions in Legionella pneumophila that attenuate intracellular infection p. 85-87. In J. M. Barbaree R. F. Breiman and A. P. Dufour (ed.) Legionella: current status and emerging perspectives. American Society for Microbiology Washington D.C.

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