Affiliation:
1. Institute for Experimental Medical Research, University of Oslo, Ullevål Hospital, Oslo, Norway
Abstract
Lymphocytes from 14 BCG-vaccinated donors, seven tuberculin positive and seven tuberculin negative by skin testing, were stimulated in vitro with four mycobacterial antigens, purified protein derivative (PPD), PPD/BCG, whole BCG bacilli, and whole
Mycobacterium leprae
and also with
Candida
antigen and phytohemagglutinin. The response was measured by incorporation of
3
H-labeled thymidine. The response to PPD, PPD/BCG, and BCG was found to correlate with the result of skin testing with turberculin. The turberculin-positive group also responded more strongly to
M. leprae
, whereas the two groups did not differ significantly in their response to
Candida
antigen or phytohemagglutinin. These findings indicate a certain degree of cross-reactivity between BCG and
M. leprae
. The use of the lymphocyte transformation test to measure antigenic cross-reactivity is discussed.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
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