Author:
Hammond G W,Lian C J,Wilt J C,Ronald A R
Abstract
Sixteen patients with clinical chancroid were studied prospectively; different culture media and sampling techniques from genital lesions were evaluated. Technique A was aspiration of a saline wash from the ulcer which was pooled and inoculated into rabbit blood, rabbit blood + vancomycin (5 microgram/ml), and semisolid chocolate agar + vancomycin (3 microgram/ml). Each primary culture medium was subcultured to chocolate agar with 1% IsoVitaleX (CA), CA with vancomycin (3 microgram/ml) plus polymyxin (7.5 microgram/ml; CA + vp). Technique B was the use of a cotton swab, plated directly on CA, CA + v, and CA + vp. Nine strains of Haemophilus ducreyi were obtained. Technique A yielded seven strains, whereas technique B yielded eight strains; with each technique, five strains were isolated only after use of selective antibiotic media. CA + v medium yielded the largest number of isolates. Direct inoculation by swab to CA + v from chancroidal ulcers is effective as an isolation technique for growth of H. ducreyi.
Publisher
American Society for Microbiology
Reference19 articles.
1. Chancroid: a report of 1402 cases;Asin J.;Am. J. Syph. Gonorrhea Vener. Dis.,1952
2. Comparison of bacteriological methods for the isolation of group B Streptococcus from vaginal cultures;Baker C. J.;J. Clin. Microbiol.,1976
3. Simplified laboratory technique for diagnosis of chancroid;Borchardt K. A.;Arch. Dermatol.,1970
4. A simplified procedure for the isolation and identification of Haemophilus ducreyi;Deacon W. E.;J. Invest. Dermatol.,1956
5. Evaluation of the ducreyi skin test for chancroid;Dienst R. B.;Am. J. Syph. Gonorrhea Vener. Dis.,1947
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