A Laboratory Exercise Simulating Antibody and Antigen Reactions of the Ouchterlony Double Immunodiffusion Assay Using Inorganic Salts
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Published:2021-09-10
Issue:2
Volume:22
Page:
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ISSN:1935-7877
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Container-title:Journal of Microbiology & Biology Education
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language:en
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Short-container-title:J Microbiol Biol Educ.
Author:
Mujtaba Mustafa G.1,
Baliban Tara1,
Bhagu Jamini1,
Herrera Michael1
Affiliation:
1. Department of Biological Sciences, Florida Gulf Coast University, Fort Myers, Florida, USA
Abstract
The Ouchterlony double immunodiffusion assay is a serological technique used in the detection of antibodies and antigens for diagnostic purposes and also used in immunology laboratory courses as a common teaching assay where students observe the geometrical precipitation line patterns that form in the agarose, elucidating degrees of homology between antigens. In this classical technique, students must wait several hours to days to obtain results when protein antigens and antibodies are used.
Publisher
American Society for Microbiology
Subject
General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,Education
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