The action of sodium deoxycholate on Escherichia coli

Author:

D'Mello A1,Yotis W W1

Affiliation:

1. Department of Microbiology, Stritch School of Medicine, Loyola University, Maywood, Illinois 60153.

Abstract

Sodium deoxycholate is used in a number of bacteriological media for the isolation and classification of gram-negative bacteria from food and the environment. Initial experiments to study the effect of deoxycholate on the growth parameters of Escherichia coli showed an increase in the lag time constant and generation time and a decrease in the growth rate constant and total cell yield of this microorganism. Cell fractionation studies indicated that sodium deoxycholate at levels used in bacteriological media interferes with the incorporation of [U-14C]glucose into the cold-trichloroacetic acid-soluble, ethanol-soluble, and trypsin-soluble cellular fractions of E. coli. Finally, sodium deoxycholate interfered with the flagellation and motility of Proteus mirabilis and E. coli. It would appear then that further improvement of the deoxycholate medium may be in order.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference11 articles.

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5. New culture medium based on sodium deoxycholate for the isolation of intestinal pathogens and for the enumeration of colon bacilli in milk and water;Leifson E.;J. Pathol. Bacteriol.,1935

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