Abstract
Extracellular vesicles of Bacteroides gingivalis (type strain 33277) were isolated, and some of their biological activities were characterized. The vesicles were obtained from a 2-day culture after ammonium sulfate precipitation, differential centrifugation, and dialysis. When viewed by electron microscopy, vesicles of approximately 50 nm predominated. The results indicated that the enriched vesicle fraction had a high proteolytic activity against collagen, Azocoll, and N-alpha-benzoyl-DL-arginine p-nitroanilide. The polypeptide pattern of the vesicles was similar but not identical to that of the outer membrane. The membrane vesicles could also promote bacterial adherence between homologous cells as well as mediate attachment between two noncoaggregating bacterial species. These vesicles could thus play an important role in periodontal diseases by serving as a vehicle for toxins and various proteolytic enzymes, as well as being involved in adherence.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference37 articles.
1. Evidence for a membrane-bound form of a bacteriocin of Bacteroides uniformis T1-1;Austin-Prather S. L.;Can. J. Microbiol.,1984
2. Organization and distribution of the cellulosome in Clostridium thermocellum;Bayer E. A.;J. Bacteriol.,1985
3. Fractionation of hemagglutinating and bacterial binding adhesins of Bacteroides gingivalis;Boyd J.;Infect. Immun.,1984
4. Brinton C. C. J. Bryan J.-A. Dillon N. Guerina L. J. Jacobson A. Labik S. Lee A. Levine S. Lim J. McMichael S. Polen K. Rogers A. C.-C. To and S. C.-M. To. 1978. Uses of pili in gonorrhea control: role of bacterial pili in disease purification and properties of gonococcal pili and progress in the development of a gonococcal pilus vaccine for gonorrhea p. 155-178. In G. F. Brooks E. C. Gotschlich K. K. Holmes W. D. Sawyer and F. E. Young (ed.) Immunobiology of Neisseria gonorrhoeae. American Society for Microbiology Washington D.C.
5. Detection of proteases by clotting of casein after gel electrophoresis;Foltmann B.;Anal. Biochem.,1985
Cited by
303 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献