Detection of type-specific antigens in the lungs of Haemophilus pleuropneumoniae-infected pigs by coagglutination test

Abstract

Specific diagnosis of Haemophilus pleuropneumoniae infection by the conventional culture and identification method usually requires 3 to 4 days. Since H. pleuropneumoniae may be disseminated from infected individuals during this period, this amount of time required for diagnosis may be too slow to aid in epidemic control. To obtain an earlier diagnosis, a coagglutination test was successfully used to detect serotype-specific antigens in lung extracts of infected pigs. A total of 19 lung tissues from experimentally infected pigs, 240 lung tissues from naturally infected pigs that died of pleuropneumonia, and 571 lung specimens from an apparently healthy pig population were examined for culture isolation as well as for antigen detection. The results showed that detection of antigens in lung tissues by the coagglutination test is an extremely rapid, simple, quite specific, and highly sensitive procedure for the diagnosis of H. pleuropneumoniae infection. Further, detection of antigens in lung tissues was found to be a much simpler and much more rapid method than culture isolation. The coagglutination test seems to be especially useful for detecting H. pleuropneumoniae in pigs exposed to chronic infection as well as those in which multiple serotypes are involved.