Author:
Faulkner G P,Shirodaria P V,Follett E A,Pringle C R
Abstract
A replicated sector-plating procedure was used to isolate 35 induced temperature-sensitive (ts) mutants and one spontaneous ts mutant from a wild-type stock of respiratory syncytial (RS) virus cloned from recent clinical material. Seven of these mutants were ts for plaque formation at 37 degrees C as well as at the restrictive temperature of 39 degrees C. The wild-type strain did not differ markedly from standard laboratory strains of RS virus. It was dependent on exogenous arginine (84 mug/ml) for optimal growth, and was not significantly inhibited by mitomycin C (10 mug/ml). It was sensitive to actinomycin D (2.5 mug/ml) during the early part of the growth phase. A characteristic focal cytopathic effect was obtained in BS-C-1 cells. Staining of infected monolayers by an indirect immunofluorescence procedure revealed a profusion of filamentous processes extending from the plasma membrane, and a similar modification of the surface of infected cells could be visualized by scanning electron microscopy. Filament production was inhibited when certain ts mutants were incubated at 39 degrees C, confirming the virus-specific nature of the phenomenon. Thirty-four of the mutants were classified into three groups by immunofluorescence. Complementation was observed in mixed infection with a single mutant from each group. Nuclear, as well as cytoplasmic, immunofluorescence was detected in RS virus-infected cells using a high-titer bovine anti-bovine RS virus serum. Visualization of nuclear antigen was dependent on the inhibition of cytoplasmic fluorescence obtained when ts mutants in groups I and III were incubated at restrictive temperature.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference20 articles.
1. Recovery from infants with respiratory illness of virus related to chimpanzee coryza agent (CCA). II. Epidemiological aspects of infection in infants and young children;Chanock R. M.;Am. J. Hyg.,1971
2. The inhibition of poliovirus growth by actinomycin-D and the prevention of the inhibition by pretreatment of the cells with serum or insulin;Cooper P. D.;Virology,1966
3. Stimulated growth of respiratory syncytial virus in the presence of actinomycin D. Antonie van Leeuwenhook J;De Jong J. C.;Microbiol. Serol.,1973
4. A study of an inhibitor in bovine serum active against respiratory syncytial virus;Doggett J. E.;Arch. Gesamte Virusforsch.,1968
5. Virus development in enucleate cells: echovirus, poliovirus, pseudorabies virus, reovirus, respiratory syncytial virus and Semliki Forest virus;Follett E. A. C.;J. Gen. Virol.,1975
Cited by
33 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献