A MecA Paralog, YpbH, Binds ClpC, Affecting both Competence and Sporulation

Author:

Persuh Marjan12,Mandic-Mulec Ines2,Dubnau David1

Affiliation:

1. Public Health Research Institute, New York, New York 10016

2. University of Ljubljana, 1000 Ljubljana, Slovenia

Abstract

ABSTRACT ComK, the master regulator of competence, is degraded by the general stress-related protease ClpCP but must be targeted to this protease by binding to the adapter protein MecA. The genome of Bacillus subtilis contains a paralog of mecA , ypbH . We show in the present study that YpbH, like MecA, binds ClpC and that its elimination or overproduction affects competence and sporulation.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference26 articles.

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3. Berka R. M. J. Hahn M. Albano I. Draskovic M. Persuh X. Cui A. Sloma W. Widner and D. Dubnau. Microarray analysis of the Bacillus subtilis K-state; genome-wide expression changes dependent on ComK. Mol. Microbiol. in press.

4. Growth medium-independent genetic competence mutants of Bacillus subtilis

5. Gottesman, S., S. Wickner, and M. R. Maurizi. 1997. Protein quality control: triage by chaperones and proteases. Genes Dev.11:815-823.

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