Anaerobic Respiration Using Fe 3+ , S 0 , and H 2 in the Chemolithoautotrophic Bacterium Acidithiobacillus ferrooxidans

Author:

Ohmura Naoya1,Sasaki Kazuhiro1,Matsumoto Norio1,Saiki Hiroshi1

Affiliation:

1. Department of Bio-Science, Central Research Institute of Electric Power Industry, 1646 Abiko, Abiko-City Chiba 270-1194, Japan

Abstract

ABSTRACT The chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans has been known as an aerobe that respires on iron and sulfur. Here we show that the bacterium could chemolithoautotrophically grow not only on H 2 /O 2 under aerobic conditions but also on H 2 /Fe 3+ , H 2 /S 0 , or S 0 /Fe 3+ under anaerobic conditions. Anaerobic respiration using Fe 3+ or S 0 as an electron acceptor and H 2 or S 0 as an electron donor serves as a primary energy source of the bacterium. Anaerobic respiration based on reduction of Fe 3+ induced the bacterium to synthesize significant amounts of a c- type cytochrome that was purified as an acid-stable and soluble 28-kDa monomer. The purified cytochrome in the oxidized form was reduced in the presence of the crude extract, and the reduced cytochrome was reoxidized by Fe 3+ . Respiration based on reduction of Fe 3+ coupled to oxidation of a c- type cytochrome may be involved in the primary mechanism of energy production in the bacterium on anaerobic iron respiration.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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