Physical Properties of Lactic Dehydrogenase-Elevating Virus and Its Ribonucleic Acid

Author:

Darnell Margo B.1,Plagemann Peter G. W.1

Affiliation:

1. Department of Microbiology, The University of Minnesota Medical School, Minneapolis, Minnesota 55455

Abstract

Infectious lactic dehydrogenase-elevating virus propagated in primary cultures of mouse peritoneal macrophages in the presence of 3 H-uridine and isolated by isopycnic centrifugation was found to have a density of 1.12 g/cm 3 . Ribonucleic acid extracted from the virus by treatment with sodium dodecyl sulfate was single stranded with a sedimentation coefficient of approximately 48 S .

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference27 articles.

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4. Separation of Newcastle disease virus proteins by polyacrylamide gel electrophoresis;Evans M. J.;Virology,1969

5. Replication of Riley's plasma enzymes elevating virus in tissue culture: the importance of the cellular composition;Evans R.;J. Gen. Virol.,1967

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