Affiliation:
1. Department of Microbiology, Oregon State University, Corvallis, Oregon 97331
2. Department of Science and Education Administration-Federal Research, U.S. Department of Agriculture, Oregon State University, Corvallis, Oregon 97331
Abstract
A microorganism that produces glucose isomerase was isolated from soil and identified as a strain of
Streptomyces flavogriseus
. The organism produced a large quantity of glucose isomerase when grown on straw hemicellulose, xylan, xylose, and H
2
SO
4
hydrolysate of ryegrass straw. The organism produced glucose isomerase both intra- and extra-cellularly. The highest level of intracellular glucose isomerase (3.5 U/ml) was obtained in about 36 h by a culture grown on straw hemicellulose; the extracellular enzyme (1.5 U/ml) appeared in cultures grown for about 72 h. About equal levels of enzyme were produced in cultures grown on straw hemicellulose, xylan, xylose, and H
2
SO
4
hydrolysate of straw, but production of the enzyme was drastically reduced when the organism was grown on other carbon sources. As a nitrogen source, corn steep liquor produced the best results. Soy flour extract, yeast extract, and various peptones also were adequate substrates for glucose isomerase production. Addition of Mg
2+
, Mn
2+
, or Fe
2+
to the growth medium significantly enhanced enzyme production. The organism, however, did not require Co
2+
, which is commonly required by microorganisms used in the production of glucose isomerase.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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