Affiliation:
1. Department of Microbiology, The Wallenberg Laboratory, Uppsala University, Uppsala, Sweden
Abstract
Denatured adenovirus DNA was retained by hydroxyapatite columns under conditions generally used for selective retention of double-stranded DNA, probably due to several partially complementary sequences within single-stranded DNA. It was found that addition of formamide reduced the fraction of sonically treated, denatured adenovirus DNA bound to hydroxyapatite from about 30% to less than 1%. This led to a study of the effect of formamide on the melting temperature (
T
m
) of double-stranded DNA in solution or bound to hydroxyapatite. The
T
m
of DNA decreases 0.56 C/1% formamide, a value determined in buffered solutions with purified formamide.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
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