Electron capture gas-liquid chromatographic-mass spectral identification of acids produced by Neisseria meningitidis in a defined medium

Author:

Alley C C,Brooks J B,Kellogg D S

Abstract

The acid metabolites and the cellular fatty acids of three strains of Neisseria meningitidis grown in a chemically defined liquid medium were determined with computerized frequency-pulsed electron capture gas-liquid chromatography. Five acids not previously reported were subsequently identified: isobutyric, octanoic, decenoic (C10:1), dodecenoic (C12:1), and tetradecenoic (C14:1). These acids were produced during active metabolism and were not detected as cellular constituents. The frequency-pulsed electron capture gas-liquid chromatography methods which we used provide a rapid, reliable, sensitive means of detecting both these and other metabolic and cellular acids in spent culture medium.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference18 articles.

1. Electron capture gas-liquid chromatography of short chain acids as their 2,2,2-trichloroethyl esters;Alley C. C.;Anal. Chem.,1976

2. Gas chromatography of bacterial whole cell methanolysates. III. Group relations of Neisseriae and;Bovre K.;Moraxellae. Acta. Pathol. Microbiol. Scand. Sect. B.,1972

3. Brooks J. B. 1975. Identification of disease and diseasecausing agents by analysis of spent culture media and body fluids with electron capture gas-liquid chromatography p. 45-54. In D. Schlessinger Microbiology-1975. American Society for Microbiology Washington D.C.

4. Detection of bacterial metabolites in spent culture media and body fluids by electron capture gas-liquid chromatography;Brooks J. B.;Adv. Chromatogr.,1977

5. Analysis by gas chromatography of fatty acids found in whole culture extracts of Neisseria species;Brooks J. B.;Can. J. Microbiol.,1971

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