Affiliation:
1. Public Health Microbiology Laboratory, Contra Costa County Health Department, Martinez, California 94553
Abstract
The rate of isolation of organisms resembling
Haemophilus vaginalis (Corynebacterium vaginale)
from vaginal specimens was not significantly affected by anaerobic versus carbon dioxide incubation atmospheres or whether specimens were inoculated on isolation media immediately after collection or after a delay of 6 h. Forty-one clinically isolated strains were provisionally divided into 30
H. vaginalis
strains and 11
H. vaginalis
-like (HVL) strains based on morphological and growth characteristics. The
H. vaginalis
strains were less reactive in API-20A identification test strips, (Analytab Products, Inc.) using Lombard-Dowell broth, than in a modified basal medium that contained proteose peptone no. 3 (Difco). The numbers and kinds of substrates fermented by 30 clinical and 2 reference strains of
H. vaginalis
varied among conventional, API, Minitek (Baltimore Biological Laboratory), and rapid buffered substrate fermentation systems. A greater number and variety of carbohydrates were fermented by the 11 HVL strains more consistently in all four test systems. Analysis of volatile and nonvolatile fermentation end products by gas-liquid chromatography did not reveal significant differences between the
H. vaginalis
and HVL strains. However, the latter group grew in peptone-yeast extract-glucose broth, whereas the
H. vaginalis
strains did not grow without the addition of starch to peptone-yeast extract-glucose. All of the reference and clinical strains were similar in their susceptibilities to a variety of antimicrobial compounds except sulfonamides, which inhibited the HVL strains and bifidobacteria but not the
H. vaginalis
strains. Sulfonamide susceptibility or resistance corresponded in part to the
H. vaginalis
and HVL-bifidobacteria strain reactions on selected conventional fermentation substrates. Susceptibility or resistance to sulfonamides and metronidazole in conjunction with fermentation tests is described to aid in the separation of
H. vaginalis
from other possibly unrecognized biotypes of
H. vaginalis
or other vaginal bacteria that presumptively resemble the organism. A human blood medium known as V agar was also of considerable value in distinguishing
H. vaginalis
from HVL strains, because only the
H. vaginalis
strains produced diffuse beta-hemolysis on V agar.
Publisher
American Society for Microbiology
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