Rapid Diagnosis of Mumps Virus Infections by Immunofluorescence Methods

Author:

Lennette David A.1,Emmons Richard W.1,Lennette Edwin H.1

Affiliation:

1. Viral and Rickettsial Disease Laboratory, California State Department of Health, Berkeley, California 94704

Abstract

Mumps and its complications, particularly meningoencephalitis, is an important disease problem, and more rapid diagnostic methods are desirable. A study was made of immunofluorescence methods for the early detection of mumps virus isolated in cell cultures, or adsorbed directly from clinical specimens onto guinea pig erythrocytes. A specific diagnosis could be made in hours to 2 or 3 days utilizing immunofluorescence methods, in contrast to about 6 days by standard methods. Details of the direct immunofluorescence methods are presented, to encourage wider application in clinical virology laboratories.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference8 articles.

1. Laboratory diagnosis of mumps by immunofluorescence on experimentally inoculated cell cultures;Bjorvatn B.;Scand. J. Infect. Dis.,1972

2. Immunofluorescence methods for detection of influenza and mumps antibodies using infected shedded allantoic cells and impression smears of organ cultures as the sources of antigens;Blaskovic P.;Arch. Gesamte Virusforsch.,1973

3. The examination of cerebrospinal fluid cells by fluorescent staining to detect mumps antigen;Boyd J. F.;Scand. J. Infect. Dis.,1973

4. Application of the method of immunofluorescence for rapid diagnosis of mumps virus;Frankova V.;J. Hyg. Epidemiol. Microbiol. Immunol.,1970

5. Henle W. E. H. Lennette and N. J. Schmidt (ed.). 1969. Mumps virus. In Diagnostic procedures for viral and rickettsial infections ch. 13. American Public Health Association Inc. New York.

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