Affiliation:
1. Section of Molecular Genetics and Microbiology, The Institute of Cell and Molecular Biology, The University of Texas at Austin, Austin, Texas
Abstract
ABSTRACT
Pseudomonas aeruginosa
is a Gram-negative opportunistic pathogen often associated with chronic infections in the lungs of individuals with the heritable disease cystic fibrosis (CF). Previous work from our laboratory demonstrated that aromatic amino acids within CF lung secretions (sputum) not only serve as carbon and energy sources but also enhance synthesis of the cell signaling molecule
Pseudomonas
quinolone signal (PQS). The present study investigates the role of the aromatic amino acid-responsive regulator PhhR in mediating these phenotypes. Transcriptome analysis revealed that PhhR controls four putative transcriptional units (
phhA
,
hpd
,
hmgA
, and
dhcA
) involved in aromatic amino acid catabolism; however, genes involved in PQS biosynthesis were unaffected. The
phhA
,
hpd
,
hmgA
, and
dhcA
promoters were mapped by primer extension, and purified His
6
-PhhR was shown to bind the
phhA
,
hpd
, and
dhcA
promoters
in vitro
by use of electrophoretic mobility shift assays. Our work characterizes a transcriptional regulator of catabolic genes induced during
P. aeruginosa
growth in CF sputum.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
41 articles.
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