A Novel Factor Isolated from Actinobacillus actinomycetemcomitans Stimulates Mouse B Cells and Human Peripheral Blood Mononuclear Cells

Author:

Jeong Soo-Jin1,Yee Sung-Tae2,Jo Wol-Soon1,Yu Seong-Ho1,Lee Sang-Hwa1,Lim Young-Jin1,Yoo Young-Hyun1,Kim Jung-Man3,Lee Jae-Dong4,Jeong Min-Ho1

Affiliation:

1. The Institute of Medical Science1 and

2. Department of Biology, Sunchon National University, Sunchon,2 Korea

3. Department of Clinical Pathology,3 Dong-A University College of Medicine, and

4. Department of Microbiology, Pusan National University,4 Pusan, and

Abstract

ABSTRACT A novel immunostimulating factor (ISTF) of Actinobacillus actinomycetemcomitans ATCC 29522 was isolated and characterized as inducing proliferation of mouse B cells and human peripheral blood mononuclear cells. This factor was isolated from the bacterial culture medium and purified by size exclusion chromatography, dye-ligand affinity chromatography, immunoaffinity chromatography using monoclonal antibodies, and preparative electrophoresis. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the purified ISTF migrated as a single band corresponding to a molecular mass of 13 kDa. ISTF was a proteinaceous material distinct from lipopolysaccharide; it directly induced the proliferation of B lymphocytes but had no effect on the proliferation of T lymphocytes, even in the presence of antigen-presenting cells. A B-lymphocyte-mitogenic activity of ISTF was also shown by flow cytometric analysis of responding cell subpopulations. Immunoblot analysis revealed that ISTF was a component of the outer membranes of bacteria, could exist as a soluble form, and was released by growing and/or lysed bacteria. These results suggest that ISTF produced by A. actinomycetemcomitans may play an important role in immunopathologic changes associated with A. actinomycetemcomitans infections.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference34 articles.

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