Development and Evaluation of a PCR Method for Detection of the Clostridium difficile Toxin B Gene in Stool Specimens-Reference-Cited by-同舟云学术

Development and Evaluation of a PCR Method for Detection of the Clostridium difficile Toxin B Gene in Stool Specimens

Published:2002-06 Issue:6 Volume:40 Page:2288-2290
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Guilbault C.¹,Labbé A.-C.¹,Poirier L.¹,Busque L.²,Béliveau C.¹,Laverdière M.¹

Affiliation:

1. Microbiology Department

2. Hematology Department, Maisonneuve-Rosemont Hospital, and University of Montreal, Montreal, Quebec, Canada

Abstract

ABSTRACT A PCR assay detecting Clostridium difficile toxin B gene in stool specimens was compared to the cytotoxicity assay as the reference standard for the diagnosis of C. difficile antibiotic-associated diarrhea (CDAD). Overall, 118 stool samples were tested. All of the specimens that were negative by the cytotoxicity assay (59 out of 118) were also negative by the PCR method (specificity of 100%). Of the 59 cytotoxin-positive samples, 54 were PCR positive (sensitivity of 91.5%). This PCR method is promising for rapid diagnosis of CDAD.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.6.2288-2290.2002

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