VirB, a Key Transcriptional Regulator of Virulence Plasmid Genes in Shigella flexneri, Forms DNA-Binding Site-Dependent Foci in the Bacterial Cytoplasm-Reference-Cited by-同舟云学术

VirB, a Key Transcriptional Regulator of Virulence Plasmid Genes in Shigella flexneri, Forms DNA-Binding Site-Dependent Foci in the Bacterial Cytoplasm

Published:2021-05-07 Issue:11 Volume:203 Page:
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Socea Jillian N.¹^ORCID,Bowman Grant R.²^ORCID,Wing Helen J.¹^ORCID

Affiliation:

1. School of Life Sciences, University of Nevada—Las Vegas, Las Vegas, Nevada, USA

2. Department of Molecular Biology, University of Wyoming, Laramie, Wyoming, USA

Abstract

This study reveals the subcellular localization of VirB, a key transcriptional regulator of virulence genes found on the large virulence plasmid (pINV) in Shigella. Fluorescent signals generated by an active GFP-VirB fusion form 2, 3, or 4 discrete foci in the bacterial cytoplasm, predominantly at the quarter-cell position. These signals are completely dependent upon VirB interacting with its DNA-binding site found either on the virulence plasmid or an engineered surrogate.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

HHS | NIH | National Institute of General Medical Sciences

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00627-20

Reference83 articles.

1. Subcellular localization of RNA and proteins in prokaryotes

2. Why and How Bacteria Localize Proteins