Isolation and characterization of a mutation that alters the substrate specificity of the Escherichia coli glucose permease-Reference-Cited by-同舟云学术

Isolation and characterization of a mutation that alters the substrate specificity of the Escherichia coli glucose permease

Published:1996-02 Issue:3 Volume:178 Page:940-942
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Begley G S¹,Warner K A¹,Arents J C¹,Postma P W¹,Jacobson G R¹

Affiliation:

1. Department of Biology, Boston University, Massachusetts 02215, USA.

Abstract

We isolated 10 mannitol-positive mutants from a mannitol-negative Escherichia coli strain. These mutations mapped within ptsG, encoding the glucose permease (EIIGlc), and resulted in a G-320-to-V substitution that allows EIIGlc to transport mannitol. Gly-320 lies within a putative transmembrane helix of EIIGlc that may be involved in substrate recognition.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.178.3.940-942.1996

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