Affiliation:
1. Communicable Disease Center, U.S. Public Health Service, Department of Health, Education, and Welfare, Atlanta, Georgia
Abstract
Kaufman, Leo
(Communicable Disease Center, Atlanta, Ga.)
and William Kaplan
. Preparation of a fluorescent antibody specific for the yeast phase of
Histoplasma capsulatum
. J. Bacteriol.
82:
729–735. 1961.—Rabbit anti-
Histoplasma capsulatum
globulin was conjugated with fluorescein isothiocyanate. This labeled antibody, in addition to staining six strains of
H. capsulatum
, exhibited strong cross-staining with ten strains of
Blastomyces dermatitidis
and numerous other species of fungi. Adsorption of the labeled antiglobulin either twice with cells of
B. dermatitidis
or twice with cells of
Coccidioides immitis
and once with cells of
B. dermatitidis
resulted in a good staining fluorescent antibody reagent, specific for yeast phase cells of
H. capsulatum
.
Attempts to define the specific labeled antibody by the Ouchterlony procedure were unsuccessful. The specific conjugate did not give a complement fixation titer with yeast phase cells of
B. dermatitidis
or with coccidioidin. It did, however, show a 1:16 complement fixation titer with homologous yeast phase cells and a weaker reaction with histoplasmin.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference10 articles.
1. Fluorescent protein tracers: The unreacted fluorescent material in fluorescein conjugates and studies of conjugates with other green fluorochromes;CHADWICK C. S.;Immunology,1960
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3. Fluorescent staining of Histoplasma capsulatum;GORDON M. A.;J. Bacteriol.,1959
4. Fluorescent antibody staining of Sporotrichum schenckii in cultures and clinical materials;KAPLAN W.;J. Invest. Dermatol.,1960
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