Evaluation of a Group-Specific 16S Ribosomal DNA-Based PCR for Detection of Helicobacter bizzozeronii, Helicobacter felis , and Helicobacter salomonis in Fresh and Paraffin-Embedded Gastric Biopsy Specimens-Reference-Cited by-同舟云学术

Evaluation of a Group-Specific 16S Ribosomal DNA-Based PCR for Detection of Helicobacter bizzozeronii, Helicobacter felis , and Helicobacter salomonis in Fresh and Paraffin-Embedded Gastric Biopsy Specimens

Published:2001-03 Issue:3 Volume:39 Page:1197-1199
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

De Groote Dominic¹,Haesebrouck Freddy¹,van Doorn Leen-Jan²,Vandamme Peter³,Ducatelle Richard¹

Affiliation:

1. Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke,1 and

2. Delft Diagnostic Laboratory, 2600 GA Delft, The Netherlands2

3. Laboratory of Microbiology, Faculty of Pharmaceutical Sciences, Ghent University, B-9000 Ghent,3 Belgium, and

Abstract

ABSTRACT A new specific and sensitive 16S ribosomal DNA-based PCR assay was developed. The assay targets a 78-bp DNA fragment unique to Helicobacter bizzozeronii, Helicobacter felis , and Helicobacter salomonis and can be used with freshly frozen and formalin-fixed paraffin-embedded gastric biopsy specimens.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.3.1197-1199.2001

Reference11 articles.

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4. A molecular scheme based on 23S rRNA gene polymorphisms for rapid identification of Campylobacter and Arcobacter species

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