Quantitation of Porcine Cytomegalovirus in Pig Tissues by PCR-Reference-Cited by-同舟云学术

Quantitation of Porcine Cytomegalovirus in Pig Tissues by PCR

Published:2001-03 Issue:3 Volume:39 Page:1155-1156
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Fryer Jacqueline F. L.¹,Griffiths Paul D.¹,Fishman Jay A.²,Emery Vincent C.¹,Clark Duncan A.¹

Affiliation:

1. Department of Virology, Royal Free and University College Medical School of UCL, London, United Kingdom,1 and

2. Transplant Infectious Disease Program, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts2

Abstract

ABSTRACT A quantitative-competitive PCR for the quantification of porcine cytomegalovirus (PCMV) was developed. The virus was detected in a variety of pig organs (including potential xenotransplant donations), with viral loads ranging from <10 to 97 genome copies/μg of DNA. This assay will have significant utility for studying the activation and replication of PCMV and in swine models for allo- and xenotransplantation.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.3.1155-1156.2001

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